Development of a radioimmunoassay for the soybean phytoalexin glyceollin I.
Identifieur interne : 002E35 ( Main/Exploration ); précédent : 002E34; suivant : 002E36Development of a radioimmunoassay for the soybean phytoalexin glyceollin I.
Auteurs : P. Moesta [Allemagne] ; M G Hahn ; H. GrisebachSource :
- Plant physiology [ 0032-0889 ] ; 1983.
Abstract
A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the tracer. A logit plot of a standard concentration series yielded a straight line in the range of 1 to 100 picomoles (0.34-34 nanograms) of glyceollin I. The structurally related pterocarpan phytoalexins, glyceollins II and III, glyceollidin II and glycinol, which also accumulate in infected soybean tissue, show a low cross-reactivity in the radioimmunoassay (0.5-5% at 50% displacement of the tracer). Two related isoflavones present constitutively in soybean tissue, daidzein and genistein, have cross-reactivities of less than 0.84% and 1.1%, respectively. The radioimmunoassay permitted the quantitative determination of glyceollin I in 15-micrometer microtome sections of soybean hypocotyl tissue infected with zoospores of Phytophthora megasperma f. sp. glycinea.
DOI: 10.1104/pp.73.2.233
PubMed: 16663200
PubMed Central: PMC1066445
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Moesta</name><affiliation wicri:level="3"><nlm:affiliation>Biologisches Institut II der Universität, Schänzlestrasse 1, D-7800 Freiburg i.Br., Federal Republic of Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Biologisches Institut II der Universität, Schänzlestrasse 1, D-7800 Freiburg i.Br.</wicri:regionArea><placeName><region type="land" nuts="1">Bade-Wurtemberg</region><region type="district" nuts="2">District de Fribourg-en-Brisgau</region><settlement type="city">Fribourg-en-Brisgau</settlement></placeName></affiliation></author><author><name sortKey="Hahn, M G" sort="Hahn, M G" uniqKey="Hahn M" first="M G" last="Hahn">M G Hahn</name></author><author><name sortKey="Grisebach, H" sort="Grisebach, H" uniqKey="Grisebach H" first="H" last="Grisebach">H. Grisebach</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="1983" type="published">1983</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the tracer. A logit plot of a standard concentration series yielded a straight line in the range of 1 to 100 picomoles (0.34-34 nanograms) of glyceollin I. The structurally related pterocarpan phytoalexins, glyceollins II and III, glyceollidin II and glycinol, which also accumulate in infected soybean tissue, show a low cross-reactivity in the radioimmunoassay (0.5-5% at 50% displacement of the tracer). Two related isoflavones present constitutively in soybean tissue, daidzein and genistein, have cross-reactivities of less than 0.84% and 1.1%, respectively. The radioimmunoassay permitted the quantitative determination of glyceollin I in 15-micrometer microtome sections of soybean hypocotyl tissue infected with zoospores of Phytophthora megasperma f. sp. glycinea.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">16663200</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>73</Volume><Issue>2</Issue><PubDate><Year>1983</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Development of a radioimmunoassay for the soybean phytoalexin glyceollin I.</ArticleTitle><Pagination><MedlinePgn>233-7</MedlinePgn></Pagination><Abstract><AbstractText>A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the tracer. A logit plot of a standard concentration series yielded a straight line in the range of 1 to 100 picomoles (0.34-34 nanograms) of glyceollin I. The structurally related pterocarpan phytoalexins, glyceollins II and III, glyceollidin II and glycinol, which also accumulate in infected soybean tissue, show a low cross-reactivity in the radioimmunoassay (0.5-5% at 50% displacement of the tracer). Two related isoflavones present constitutively in soybean tissue, daidzein and genistein, have cross-reactivities of less than 0.84% and 1.1%, respectively. 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Grisebach</name><name sortKey="Hahn, M G" sort="Hahn, M G" uniqKey="Hahn M" first="M G" last="Hahn">M G Hahn</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Moesta, P" sort="Moesta, P" uniqKey="Moesta P" first="P" last="Moesta">P. Moesta</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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